Skip to content
Da vinci european bio bank

Da vinci european bio bank

Firenze Biobank

Menu
  • Home
  • Elisa Kits
  • DNA
  • RNA
  • precipitation
  • Premix
  • Preps
  • primers
  • probe
  • E coli
  • peroxidase
  • EIA
  • electrophoresis
  • plex
  • profile
  • Purification
  • Positive
  • profiling
  • Pure
  • purified
  • Contact Us
  • Distributors
Menu
davincieuropeanbiobank

Evaluation of Abbott Anti-SARS-CoV-2 CMIA IgG and Euroimmun ELISA IgG/IgA Assays

Posted on October 27, 2020 by Gabrielle

Evaluation of Abbott Anti-SARS-CoV-2 CMIA IgG and Euroimmun ELISA IgG/IgA Assays In a Clinical Lab

Background: We evaluated the take a look at efficiency specializing in specificity of a totally automated Abbott Architect anti-SARS-CoV-2 CMIA IgG and Euroimmun anti-SARS-CoV-2 ELISA IgA/IgG in human plasma.

 

Methods: We examined constructive cohort of 97 samples from COVID-19 sufferers or healthcare staff, collected at late time factors from symptom onsets. We additionally included one other cohort of 215 samples as detrimental controls, 78 of which had constructive serology take a look at outcomes of different infectious ailments or autoimmunity.

Assay specificity was assessed through the use of a complete of 847 anonymized samples which had been collected earlier than the COVID-19 pandemic from native affected person populations looking for scientific take care of rheumatoid ailments, thyroid most cancers, and therapeutic drug monitoring.

 

Results: Abbott IgG, Euroimmun IgG/IgA had excessive precision, demonstrated by each intra- and inter-day CVs of <2%. There was no Abbott or Euroimmun IgG assay cross reactivity within the 78 samples with constructive serology of non-SARS-CoV-2 infectious ailments and constructive autoimmune antibodies. The Abbott IgG has specificity of 99.6%, whereas Euroimmun IgG and IgA had been as excessive as 91.5% and 71.5%, respectively.

 

Conclusions: our analysis confirmed excessive specificity of the Abbott IgG assay, whereas it was decrease for Euroimmun IgG. Euroimmun IgA has suboptimal specificity which can restrict its scientific use. Assay sensitivity was excessive for each Abbott and Euroimmun IgG assays.

 

Distance-based paper/PMMA built-in ELISA-chip for quantitative detection of immunoglobulin G

 

The enzyme-linked immunosorbent assay (ELISA) is one of probably the most generally carried out scientific diagnostic instruments for the detection and quantification of protein biomarkers. However, typical ELISA assessments require subtle infrastructure, costly reagents, lengthy assay time, and experience for operation, which aren’t typically simply accessible in resource-limited settings.

Microfluidic ELISA-chip based mostly point-of-care (POC) testing permits miniaturization and integration of advanced capabilities that facilitate their utilization in limited-resource settings. The present work demonstrates a easy, moveable, low price and equipment-free paper/poly(methyl methacrylate) (PMMA) built-in microfluidic ELISA-chip as a POC machine with a visible distance-based readout for quantitative detection of scientific biomarkers.

The built-in paper/PMMA ELISA-chip makes use of the motion of immunoassay complexes with magnetic beads by a everlasting magnet in a PMMA half of the compartment. The goal focus is translated into a visible distance sign readout for quantitative detection of biomarkers in a μPAD.

Because it doesn’t require subtle devices and has the added benefits of low price, simple operation, and disposability with quantitative visible readout, the paper/PMMA ELISA-chip holds nice promise for moveable detection of goal bioanalytes as a POC diagnostic instrument in resource-limited setups.

 

A reference measurement of circulating ATPase inhibitory issue 1 (IF1) in people by LC-MS/MS: Comparison with typical ELISA

ATPase inhibitory issue 1 (IF1) is a 9.5 kDa protein that binds to mitochondrial and plasma membrane ATP synthase and selectively inhibits ATP hydrolysis. Recently, IF1 was recognized in systemic circulation in people. IF1 appeared as an unbiased determinant of HDL-cholesterol with decrease ranges in coronary coronary heart illness (CHD) sufferers. Moreover, IF1 was additionally discovered to negatively affiliate with mortality in these sufferers, supporting the notion that circulating IF1 may very well be a promising biomarker of heart problems.

However, in earlier research, IF1 was quantified by a non-standardized aggressive enzyme-linked immunosorbent assay (ELISA). Herein, we’ve got validated a liquid chromatography-tandem mass spectrometry technique (LC-MS/MS) enabling the correct quantification of IF1 in human plasma. Plasma IF1 was trypsin-digested by way of an optimized process earlier than LC-MS/MS evaluation.

The technique was efficiently validated over four unbiased experiments into the vary of 100-1500 ng/mL. Intra- and inter-assay variation coefficients had by no means exceeded 14.2% and accuracy ranged between 95% and 102% for the chosen EAGGAFGK peptide marker.

Subsequently, the outcomes of the LC-MS/MS technique had been in contrast with these obtained utilizing ELISA in 204 people from the GENES research. We discovered that IF1 plasma ranges obtained utilizing each methods had been strongly correlated (r = 0.89, p < 0.0001), whereas the Bland-Altman plot didn’t point out any main statistically important variations. To clinically validate LC-MS/MS, we confirmed the constructive correlation between IF1 plasma ranges and HDL-cholesterol (r = 0.38, p < 0.0001).

Besides, we discovered decrease IF1 plasma ranges in CHD sufferers in comparison with controls (431 ± 132 ng/mL and 555 ± 173 ng/mL, respectively; p < 0.0001). Hence, it may be concluded that the introduced LC-MS/MS analytical technique supplies a extremely particular technique for IF1 quantification in human plasma and may very well be proposed as a reference technique.

davincieuropeanbiobank
davincieuropeanbiobank

Detection of latent kinds of Mycobacterium avium subsp. paratuberculosis an infection utilizing host biomarker-based ELISAs significantly improves paratuberculosis diagnostic sensitivity

 

  • Bovine paratuberculosis (PTB) is a power granulomatous enteritis, attributable to Mycobacterium avium subsp. paratuberculosis (MAP), chargeable for necessary financial losses within the dairy trade. Current diagnostic strategies have low sensitivities for detection of latent kinds of MAP an infection, outlined by focal granulomatous lesions and scarce humoral response or MAP presence.

 

  • In distinction, patent infections correspond to multifocal and diffuse sorts of enteritis the place there’s elevated antibody manufacturing, and substantial mycobacterial load.
  • Our earlier RNA-Seq evaluation allowed the choice of 5 candidate biomarkers overexpressed in peripheral blood of MAP contaminated Holstein cows with focal (ABCA13 and MMP8) and diffuse (FAM84A, SPARC and DES) lesions vs. management animals with no detectable PTB-associated lesions in gut and regional lymph nodes.
  • The intention of the present research was to evaluate the PTB diagnostic potential of industrial ELISAs designed for the precise detection of these biomarkers. The capacity of these ELISAs to determine animals with latent and/or patent kinds of MAP an infection was investigated utilizing serum from naturally contaminated cattle (n = 88) and non-infected management animals (n = 67).
  • ROC evaluation revealed that the ABCA13-based ELISA confirmed the best diagnostic accuracy for the detection of contaminated animals with focal lesions (AUC 0.837, sensitivity 79.25% and specificity 88.06%) and with any kind of histological lesion (AUC 0.793, sensitivity 69.41% and specificity 86.57%) bettering on the diagnostic efficiency of the favored IDEXX ELISA and different typical diagnostic strategies.

 

  • SPARC and MMP8 confirmed the best diagnostic accuracy for the detection of animals with multifocal (AUC 0.852) and diffuse lesions (AUC 0.831), respectively. In conclusion, our outcomes recommend that quantification of ABCA13, SPARC and MMP8 by ELISA has the potential for implementation as a diagnostic instrument to reliably determine MAP an infection, significantly bettering early detection of MAP latent infections when antibody responses and fecal shedding are undetectable utilizing typical diagnostic strategies.

 

 

 

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

next day delivery

[email protected]

Recent Posts

  • Compare Pcr lab reagents for research
  • Compare antibodies lab reagents for research
  • 17-OHP(17-Hydroxyprogesterone) ELISA Kit
  • The value of virtual biobanks for transparency purposes with respect to reagents and samples used during test development and validation
  • Advancing human genetics research and drug discovery through exome sequencing of the UK Biobank

Meta

  • Log in
  • Entries feed
  • Comments feed
  • WordPress.org

Clinical_Bioche Follow

clinical_bioche Clinical and Medical Biochemistry Journal @clinical_bioche ·
2 Feb

#Chronic_obstructive pulmonary disease, or COPD, refers to a group of #diseases that cause airflow #blockage and breathing-related problems.

Reply on Twitter 1621125155958439936 Retweet on Twitter 1621125155958439936 Like on Twitter 1621125155958439936 1 Twitter 1621125155958439936
Retweet on Twitter Clinical and Medical Biochemistry Journal Retweeted
jamaicanlabrat 🥂👩‍🔬 Not Actinomagnificent @jamaicanlabrat ·
14 Jan 2019

I think I need safer initials 🤔

Reply on Twitter 1084884786362109955 Retweet on Twitter 1084884786362109955 31 Like on Twitter 1084884786362109955 252 Twitter 1084884786362109955
Retweet on Twitter Clinical and Medical Biochemistry Journal Retweeted
thecopperdoctor Karrera Djoko @thecopperdoctor ·
17 Nov

My group is looking to recruit not 1, not 2, but 3 (yes, three!) PhD students interested in metals and host-microbe interactions. Guaranteed funding available for 1 UK candidate, competitive funding available for 2 international candidates. #FindAPhD

Reply on Twitter 1593241657222103040 Retweet on Twitter 1593241657222103040 418 Like on Twitter 1593241657222103040 662 Twitter 1593241657222103040
Retweet on Twitter Clinical and Medical Biochemistry Journal Retweeted
doudna_lab Doudna Lab @doudna_lab ·
23 Jan

Brace yourself… More robust than RNAi and less toxic than Cas13… check out our latest story on CRISPR-Csm for precise RNA targeting!!

@NatureBiotech @igisci

https://www.nature.com/articles/s41587-022-01649-9

Reply on Twitter 1617617130652053506 Retweet on Twitter 1617617130652053506 599 Like on Twitter 1617617130652053506 2345 Twitter 1617617130652053506
clinical_bioche Clinical and Medical Biochemistry Journal @clinical_bioche ·
18 Jan

#Crohn's #disease is a type of inflammatory bowel disease (IBD). It causes swelling of the #tissues (inflammation) in your #digestive tract

Reply on Twitter 1615687290726281221 Retweet on Twitter 1615687290726281221 1 Like on Twitter 1615687290726281221 2 Twitter 1615687290726281221
Load More

Categories

  • Bio-Fabrication: Convergence of 3D Bioprinting and Nano-Biomaterials in Tissue Engineering and Regenerative Medicine.
  • Creating Complex Fluorophore Spectra on Antibodies Through Combinatorial Labeling.
  • DNA
  • E coli
  • EIA
  • electrophoresis
  • Elisa Kits
  • Fibrillar Collagen Quantification With Curvelet Transform Based Computational Methods.
  • High fat diet decreases beneficial effects of estrogen on serotonin-related gene expression in marmosets.
  • Inducible ASABF-type antimicrobial peptide from the sponge Suberites domuncula: microbicidal and hemolytic activity in vitro and toxic effect on molluscs in vivo.
  • peroxidase
  • plex
  • Positive
  • precipitation
  • Premix
  • Preps
  • primers
  • probe
  • profile
  • profiling
  • Pure
  • Purification
  • purified
  • RNA
  • The scientific literature on Coronaviruses, COVID-19 and its associated safety-related research dimensions: A scientometric analysis and scoping review.

Tags

antibody dna dnajlion7 dnajlion7 youtube dnalion7 youtube dna testing sites dna testing song dna testing while pregnant dna tests elisa kits equipments cleary equipments data equipments fds equipments gym equipmentshare careers equipmentshare ceo equipmentshare controller equipment shunned by fly fishers equipment shunned by fly fishers crossword equipments mtg equipments needed in building an aquarium equipment synonym equipment trader peptides for erectile dysfunction proteins rna rna-seq rnac rnahybrid rnai rna reset rnascope rna sequence rna sequencing rna splicing rna structure rnation.riteaid.com rnation login rna vaccine rna vaccine wiki rna velocity rna viruses rna vs dna rna world test kits cdc

next day delivery

[email protected]

Recent Posts

  • Compare Pcr lab reagents for research
  • Compare antibodies lab reagents for research
  • 17-OHP(17-Hydroxyprogesterone) ELISA Kit
  • The value of virtual biobanks for transparency purposes with respect to reagents and samples used during test development and validation
  • Advancing human genetics research and drug discovery through exome sequencing of the UK Biobank

Meta

  • Log in
  • Entries feed
  • Comments feed
  • WordPress.org

Clinical_Bioche Follow

clinical_bioche Clinical and Medical Biochemistry Journal @clinical_bioche ·
2 Feb

#Chronic_obstructive pulmonary disease, or COPD, refers to a group of #diseases that cause airflow #blockage and breathing-related problems.

Reply on Twitter 1621125155958439936 Retweet on Twitter 1621125155958439936 Like on Twitter 1621125155958439936 1 Twitter 1621125155958439936
Retweet on Twitter Clinical and Medical Biochemistry Journal Retweeted
jamaicanlabrat 🥂👩‍🔬 Not Actinomagnificent @jamaicanlabrat ·
14 Jan 2019

I think I need safer initials 🤔

Reply on Twitter 1084884786362109955 Retweet on Twitter 1084884786362109955 31 Like on Twitter 1084884786362109955 252 Twitter 1084884786362109955
Retweet on Twitter Clinical and Medical Biochemistry Journal Retweeted
thecopperdoctor Karrera Djoko @thecopperdoctor ·
17 Nov

My group is looking to recruit not 1, not 2, but 3 (yes, three!) PhD students interested in metals and host-microbe interactions. Guaranteed funding available for 1 UK candidate, competitive funding available for 2 international candidates. #FindAPhD

Reply on Twitter 1593241657222103040 Retweet on Twitter 1593241657222103040 418 Like on Twitter 1593241657222103040 662 Twitter 1593241657222103040
Retweet on Twitter Clinical and Medical Biochemistry Journal Retweeted
doudna_lab Doudna Lab @doudna_lab ·
23 Jan

Brace yourself… More robust than RNAi and less toxic than Cas13… check out our latest story on CRISPR-Csm for precise RNA targeting!!

@NatureBiotech @igisci

https://www.nature.com/articles/s41587-022-01649-9

Reply on Twitter 1617617130652053506 Retweet on Twitter 1617617130652053506 599 Like on Twitter 1617617130652053506 2345 Twitter 1617617130652053506
clinical_bioche Clinical and Medical Biochemistry Journal @clinical_bioche ·
18 Jan

#Crohn's #disease is a type of inflammatory bowel disease (IBD). It causes swelling of the #tissues (inflammation) in your #digestive tract

Reply on Twitter 1615687290726281221 Retweet on Twitter 1615687290726281221 1 Like on Twitter 1615687290726281221 2 Twitter 1615687290726281221
Load More

RSS Biotechnology

  • Movember Mese di sensibilizzazione sul cancro maschile
    The post Movember Mese di sensibilizzazione sul cancro maschile appeared first on Gentaur Italia.
  • Che cos’è il vaiolo delle scimmie (Monkeypox): “Aumento significativo dei casi in Europa e negli Stati Uniti”.
    The post Che cos’è il vaiolo delle scimmie (Monkeypox): “Aumento significativo dei casi in Europa e negli Stati Uniti”. appeared first on Gentaur Italia.
  • Stealth Omicron: cos’è la sottovariante Omicron BA.2 a rapida diffusione?
    The post Stealth Omicron: cos’è la sottovariante Omicron BA.2 a rapida diffusione? appeared first on Gentaur Italia.
  • Omicron notizie : mezza Europa sarà infettata da omicron in breve tempo
    The post Omicron notizie : mezza Europa sarà infettata da omicron in breve tempo appeared first on Gentaur Italia.
  • Notizie : test PCR attivati dalla luce
    The post Notizie : test PCR attivati dalla luce appeared first on Gentaur Italia.
  • Allarme come 170 casi di “flurone” rilevato in Inghilterra, flurone covid
    The post Allarme come 170 casi di “flurone” rilevato in Inghilterra, flurone covid appeared first on Gentaur Italia.
  • Puntali con filtro PCR: cosa sono e come utilizzarli
    The post Puntali con filtro PCR: cosa sono e come utilizzarli appeared first on Gentaur Italia.
  • Cosa è un One Step RT-qPCR Master Mix: guida completa
    The post Cosa è un One Step RT-qPCR Master Mix: guida completa appeared first on Gentaur Italia.
  • Nuova variante Omicron: quali sono le ultime notizie?
    The post Nuova variante Omicron: quali sono le ultime notizie? appeared first on Gentaur Italia.
  • Nuova variante Covid-19 omicron: cosa sappiamo?
    The post Nuova variante Covid-19 omicron: cosa sappiamo? appeared first on Gentaur Italia.

Categories

  • Bio-Fabrication: Convergence of 3D Bioprinting and Nano-Biomaterials in Tissue Engineering and Regenerative Medicine.
  • Creating Complex Fluorophore Spectra on Antibodies Through Combinatorial Labeling.
  • DNA
  • E coli
  • EIA
  • electrophoresis
  • Elisa Kits
  • Fibrillar Collagen Quantification With Curvelet Transform Based Computational Methods.
  • High fat diet decreases beneficial effects of estrogen on serotonin-related gene expression in marmosets.
  • Inducible ASABF-type antimicrobial peptide from the sponge Suberites domuncula: microbicidal and hemolytic activity in vitro and toxic effect on molluscs in vivo.
  • peroxidase
  • plex
  • Positive
  • precipitation
  • Premix
  • Preps
  • primers
  • probe
  • profile
  • profiling
  • Pure
  • Purification
  • purified
  • RNA
  • The scientific literature on Coronaviruses, COVID-19 and its associated safety-related research dimensions: A scientometric analysis and scoping review.

Recent Posts

  • Compare Pcr lab reagents for research
  • Compare antibodies lab reagents for research
  • 17-OHP(17-Hydroxyprogesterone) ELISA Kit
  • The value of virtual biobanks for transparency purposes with respect to reagents and samples used during test development and validation
  • Advancing human genetics research and drug discovery through exome sequencing of the UK Biobank
© 2023 Da vinci european bio bank | Powered by Superbs Personal Blog theme